Journal: eBioMedicine

Article Title: Neural synaptic vesicle autoimmunity following aerosolized porcine neural tissue exposure: insights into autoimmune inflammatory polyradiculoneuropathy

doi: 10.1016/j.ebiom.2025.106053

Figure Lengend Snippet: Confirmation of synaptophysin- and GAP43-IgG in patients with OIPN by ELISA and CBA . a) Fifteen of 20 patients with OIPN sera tested positive for synaptophysin-IgG by ELISA compared to none of the control cohorts. b) Representative immunofluorescent images of IgG binding to GFP-tagged synaptophysin-transfected COS7 cells. Commercial synaptophysin-IgG and OIPN-IgG (magenta) co-localises (white in merge) with GFP-tagged synaptophysin protein (green). c) Twelve of 20 sera from patients with OIPN tested positive for GAP43-IgG by ELISA. d) Representative immunofluorescent images of IgG binding (magenta) to GFP-tagged GAP43 transfected COS7 cells. Colocalisation (white in merge) is observed for commercial antibody and OIPN-IgG with GFP-GAP43 protein. b and d) DNA is labelled with DAPI, blue. Healthy adult human IgG fails to bind to transfected cells. Note: No IgG bound to non-transfected cells (solitary blue nuclei). Scale bars, 20 μm. Key: OIPN, occupational inflammatory polyradiculoneuropathy; ELISA, enzyme-linked immunosorbent assay; GFP, green fluorescence protein; DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Immulon 2HB plates were coated (10 ng/well) with PhIP-Seq-identified antigens: synaptophysin peptide 224-313 (Mayo Clinic Proteomics Core Facility) or GAP43 recombinant protein (Novus, #NBP2-53033) diluted in 0.01 M PBS, pH 7.4.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Binding Assay, Transfection, Fluorescence

Journal: Investigative ophthalmology & visual science

Article Title: Three-Dimensional Neurite Characterization of Small Incision Lenticule Extraction Derived Lenticules.

doi: 10.1167/iovs.19-27566

Figure Lengend Snippet: FIGURE 4. Interaction between lenticule neurites and SCs. Colocaliza- tion of SC marker expression and stromal neurites by confocal immunofluorescence: (A–C) GAP43 (green) and TuJ1 (red). (D–F) p75NTR (green) and TuJ1 (red).

Article Snippet: Antibodies Used in This Study Antibody Source Working Concentration 1 bIII-tubulin Covance MMS435P 0.5 lg/mL 2 GAP43 Novus NB300-143 0.5 lg/mL 3 P75NTR Millipore AB1554 0.5 lg/mL 4 Phalloidin-AlexaFluor 594 conjugate Invitrogen 0.5 lg/mL 5 AlexaFluor 488 goat anti-mouse/rabbit IgG (HþL) Jackson ImmunoRes Lab Immunostaining: 1:700 6 RedX-conjugated goat anti-mouse/rabbit IgG (HþL) Jackson ImmunoRes Lab Immunostaining: 1:700 Downloaded from iovs.arvojournals.org on 10/24/2019 placed as flat-mount.

Techniques: Marker, Expressing

Journal: Journal of Neuroscience

Article Title: Toll-Like Receptor Signaling Is Critical for Wallerian Degeneration and Functional Recovery after Peripheral Nerve Injury

doi: 10.1523/jneurosci.3027-07.2007

Figure Lengend Snippet: Figure 3. Deficiency in TLR signaling compromises macrophage recruitment/activation and delays myelin debris clearance, axonal regeneration, and locomotor recovery after sciatic nerve lesion. A–D, Representative fluorescence photomicrographs takenfromlongitudinalsectionsofthesciaticnervedistaltothelesionshowingCD68-immunopositivemacrophagesinC57BL/6J (A),TLR2-ko(B),C3H/HeOUJ(C),andTLR4d(D)miceat7d.E,QuantificationofthenumberofCD68macrophagesinthesciaticnerve distalstumpofTLR2-ko,TLR4d,MyD88-ko,andwild-typemiceat7dafterlesion(n8pergroup).F–I,Bright-fieldphotomicrographs showingmyelinstainedwithLFBinthesciaticnervedistalstumpofC57BL/6J(F),TLR2-ko(G),C3H/HeOUJ(H),andTLR4d(I)miceat7d. J,QuantificationofLFBstainingofmyelininthedegeneratingsciaticnervedistalstumpofmicedeficientinTLRsignalingat7d(n8per group). K, Quantification of the number of axons that had regenerated up to 4 mm distal to the site of lesion, as visualized by GAP-43 immunofluorescence, at 4 d after lesion. L, M, Recovery of locomotor functions from a sciatic nerve lesion, as determined by the SFI, in TLR2-ko,MyD88-ko,andTLR4dmicecomparedwiththeirrespectivecontrolgroups(WT)overaperiodof49d(n12pergroup).***p 0.001,**p0.01,and*p0.05comparedwiththecontrolgroup.Scalebar,50m.

Article Snippet: Immunohistochemistry was performed to detect the following antigens: (1) mouse CD68 using the monoclonal anti-CD68 antibody (to visualize activated macrophages/monocytes; Serotec, Raleigh, NC), (2) growth-associated protein-43 (GAP-43) using the polyclonal antiGAP-43 antibody (to visualize regenerating axons; Novus Biologicals, Littleton, CO), (3) rat CD68 using the monoclonal ED-1 antibody (to visualize activated macrophages/monocytes; Serotec), (4) ionized calcium-binding adaptor molecule 1 (iba1) using the polyclonal antiiba1 antibody (to visualize macrophages/monocytes; Wako Chemicals USA, Richmond, VA), and (5) mouse CD45 using the monoclonal antiCD45 antibody (to visualize leukocytes; BD PharMingen, Mississauga, Ontario, Canada).

Techniques: Activation Assay, Fluorescence, Immunofluorescence